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Fig. 2. Anterior overexpression of FGF8 causes prolonged downregulation of WNT
expression in the hem, and a defective hippocampus. (A-L) Coronal sections of
E15.5 brains processed for in situ hybridization. Medial is towards the left.
Brains are electroporated with Ap (A-C,G-I) or Fgf8
(D-F,J-L) at E11.5. Each row illustrates three near-adjacent sections from the
same brain. (A-C) A brain electroporated with Ap (transgene in B)
shows normal expression of Wnt3a at the hem remnant (arrow, A), and a
dense patch of Ka1 in the developing hippocampal CA3 field (arrow,
C). A brain electroporated with Fgf8 (E) shows a little
Wnt3a expression (D), and diffuse Ka1 expression in the
hippocampus (asterisk, F). (G-L) Similarly a brain electroporated with
Ap displays robust Wnt5a expression in the distal region of
the hippocampus (G, asterisk), compared with very sparse and diffuse
expression in a brain electroporated with Fgf8 (J, asterisk).
Prox1 marks the developing DG in I, but not in L. Scale bar: 0.25
mm.
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