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Fig. 3. Similar changes in Fgf8 and Wnt3a expression by BMP
inhibition or loss of EMX2. (A-I) E10.5 forebrains viewed dorsally, anterior
towards the top, processed for whole-mount in situ hybridization.
EGFP alone (A,D,G) or EGFP and Nog together (B,E,H)
were electroporated into the telencephalic vesicle at E9.5. EGFP fluorescence
indicates the position of the electroporated site (insets). Brains
electroporated with EGFP alone show wild-type expression patterns of
Fgf8, Wnt3a and Msx2 (a reporter of BMP activity).
Co-electroporation of Nog results in inhibition of BMP activity,
reflected in decreased Msx2 expression (arrow in H), increased and
ectopic Fgf8 expression in the cortical primordium (B), and lowered
expression of Wnt3a in the cortical hem (arrow in E). The forebrains
of Emx2 homozygote mutant mice are not identical to
Nog-electroporated brains, but, like the latter, show expanded
Fgf8 expression (C, arrow) and reduced expression of Wnt3a
in the hem (F, arrow). A slight decrease in Msx2 expression is most
evident in the cortical hem (I, arrow). The latter region is buried in a dense
mass of Msx2 expression in control forebrains (G). Scale bar: 0.4
mm.
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