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Fig. 6. Substantial rescue of WNT gene expression in the Emx2 mutant cortical hem. (A-F) E13.5 hemispheres viewed from the medial side. Wild-type expression of Wnt3a and Wnt2b (A,D). (B,C,E,F) Hemispheres electroporated anteriorly with sFGFR3c at E.9.5. Each brain was co-electroporated with EGFP. WNT gene expression remains depleted in Emx2 mutant brains with sparse or undetected EGFP (inefficiently transfected) (B,E), but expression is substantially restored in two brains that were efficiently transfected with sFGFR3c, assessed by high-density EGFP fluorescence (C,F). Scale bar: 0.4 mm.





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