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Fig. 6. BMP inhibition by Smad6 is not sufficient for neural induction in
Xenopus ventral epidermis. (A-I) cSmad6 was targeted to the
ventral epidermis by injection into the A4 blastomere of 32-cell stage embryos
(A). Smad6 does not activate Sox3 expression (B-E), compare
with GFP-injected control embryos (F-I). B,C,F,G are dorsal views;
D,E,H,I are ventral views of the embryos in B,C,F,G. C,E,G,I show the embryos
in B,D,F,H after staining with anti-fluorescein to reveal the lineage tracer
FDX which was co-injected with the mRNA. (J-L) To test that the progeny of the
A4 blastomere is competent to respond to neural inducing signals from the
organizer, a lineage tracer (FDX) was injected into the A4 blastomere. At late
blastula/early gastrula stage, the labelled cells are transplanted into the
dorsal side of an unlabelled host embryo, which is grown to early neurula
stage (J). (K,L) The transplanted cells (FDX in brown in L) contribute to the
neural plate and express Sox3 (purple).
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