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Fig. 7. Temporal and spatial regulation of tsh repression by Pc.
(A) Time line of clone induction. Red bars indicate windows in which
Pc- clones were induced by heat shock relative to hours
after egg laying (AEL) and tsh repression. Note that the 72-84 and
84-96 hours AEL time points occur after the initiation of tsh
repression in the second instar. (B-M) tsh expression (red) in
Pc– clones, marked by the absence of GFP. (B,C)
Pc– clones induced 84-96 hours AEL express
tsh in the most distal cells of the hinge (white arrow), but not in
the wing pouch. (D,E) Pc– clones induced 72-84 hours
AEL express tsh in the wing pouch and distal hinge (white box,
enlarged). (F,G) tsh expression levels in
Pc– clones induced 48-60 hours AEL are comparable to
endogenous hinge and notum levels. (H,I) Large Pc–
clones induced 24-36 hours AEL. Although tsh is widely expressed in
the mutant tissue, it is not expressed in cells close to the DV boundary
(white box, enlarged) or PH (yellow arrowhead). (J-M)
Pc– MARCM clones, marked by GFP, induced in early
second instar larvae. (J,K) Control Pc– clones
de-repress tsh. (L,M) Pc– clones expressing
Nrt-Wg do not de-repress tsh.
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