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Fig. 3. sal is required for the correct expression of R3/R4 specification
and polarity markers. All panels represent third instar eye discs where
sal– clones were induced by Flipase-mediated mitotic
recombination and are labeled by the absence of ubi-GFP staining (green).
Anterior is to the left and the equator is at the top. The blue channel shows
Ro in A and ELAV in all other panels. (A) BarH1 (red) stains R1/R6 and allows
the visualization of the progressive ommatidial rotation. White and yellow
bars indicate ommatidia with correct and incorrect rotation, respectively. (B)
In wild-type tissue, Fmi (red) localizes in the equatorial side of R3 and R4
(arrows). In sal– ommatidia, Fmi is present in all
sides of the apical membrane of R3 and R4 (arrowheads). High magnification
images of sal– (top, right) and wild-type (bottom,
right) ommatidia (asterisk) show the localization of Fmi in the R3 and R4
apical membrane (dashed line). (C) Dl expression (red), visualized
with the enhancer trap line Dl-lacZ1282, is transiently upregulated
in R3 (arrows) in two to three rows. In sal– tissue,
most ommatidia show low levels of Dl expression in both cells of the
R3/R4 pair (arrowheads). In some ommatidia, the cell in the R4 position has
stronger staining than R3 (+), or both cells in the pair have high levels of
Dl expression (asterisk). (D) The expression of
m
0.5-lacZ (red) in R4 is lost in 91% (n=218)
of sal– ommatidia. Some residual expression is still
observed in 9% of the cases. In mosaic ommatidia where R3 but not R4 (arrows,
n=29), or R4 but not R3 (arrowhead, n=21) is
sal–, m0.5-lacZ expression
is reduced. (E) The expression of svp-lacZ (red) is lost in R3/R4,
but not R1/R6, in sal– clones. Scale bars: 10
µm.
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