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Fig. 1. Reduced zones of hypertrophic chondrocytes in
Fosl2–/– embryos and newborn mice. (A)
Skeletal staining of a Fosl2–/– newborn and
wild-type littermate at P0. (B) Length of mineralized regions of knock-out and
wild-type littermates at P0; bars represent mean value±s.e.m.;
n=4. (C) Genotyping-PCR of DNA from wild-type, heterozygous and
Fosl2-null newborn mice. (D) Expression of Fosl2 in primary
rib cage chondrocytes and its absence in mutant cells. Primary chondrocytes
were cultured for 3 days prior to RNA isolation and RNase protection assay.
GAPDH was used as loading control. (E) Real-time PCR of cartilage markers.
Relative expression of type X collagen (colX), aggrecan
(agg) and Ihh is shown. Expression levels were normalized to
tubulin expression. The mean of two independent measurements is shown. (F) In
situ hybridization on sections of embryonic and postnatal femoral growth
plates of Fosl2–/– mice and littermate
controls using a type X collagen antisense probe as a marker for hypertrophic
zones. Pictures were taken at 200x (E13.5), 100x (E14.5, E16.5,
E18.5) and 50x (P2, P4) magnification.
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