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Fig. 3. Delayed development of vertebral columns in Fosl2–/– embryos. (A) Hematoxylin and Eosin staining, in situ hybridization using type II collagen (col II) and type X collagen (col X) antisense probes, and Ki67 immunohistochemistry on transversally sectioned primordial arches of developing vertebrae at E14.5. Arrowheads indicate Ki67-stained cells. (B) Hematoxylin and Eosin staining, in situ hybridization using osteocalcin (oc, arrowheads) antisense probe, Ki67 immunohistochemistry and von Kossa staining on transversally sectioned primordial arches of developing vertebrae at E16.5. Arrowheads indicate Ki67-stained cells. Pictures were taken at 100x (Haematoxylin and Eosin) and 50x (in situ hybridization, Ki67 immunohistochemistry, von Kossa staining) magnification.





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