|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Impaired differentiation and delayed senescence of primary
Fosl2–/– rib cage chondrocytes. (A) Formation
of cartilage nodules by primary chondrocytes. Primary chondrocyte cultures
stained with Alcian Blue (left panel). Quantification of Alcian Blue bound to
sulfated proteoglycans in the chondrocyte ECM (right panel). Bars represent
mean value±s.d.; n=4; *P<0.05. (B)
Cumulative cell number assay of primary rib cage chondrocytes. Experiments
were carried out in triplicate and repeated three times. One representative
experiment is shown. (C) BrdU-labeling of proliferating primary chondrocytes
after 2 days in culture. (D) TUNEL staining of apoptotic primary chondrocytes
after 2 days in culture. (E) Semi-quantitative RT-PCR analysis of cartilage
markers and Fosl2 in primary rib cage chondrocytes at day 3, 6 and 9
of differentiation. Tubulin was used as a loading control (n=3).
|
