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Fig. 6. Foxg1 (BF-1) expression is expanded, and Zic2 and Islet1 zones are reduced in the Foxd1 (BF-2)-deficient ventral diencephalon. (A) Top left panel is a dorsal view of an E15.5 embryo head with dashed red lines (1, 2) indicating the plane and level of section shown in panels a-h. Top right panel is a drawing of a sagittal section; red-dashed square indicates the area shown in panels a-h. (a-h) Sagittal cryosections of E15.5 Foxd1^lacZ/lacZ (b,d,f,h) and Foxd1^+/+ embryos (a,c,e,g), hybridized for Foxg1 (a-d), or immunostained (e-h) against Zic2 (green) and Islet1 (red). Dashed yellow lines indicate where the axons are located, in different positions in the Foxd1^+/+ embryo compared with in the Foxd1 deficient embryo. The light blue-dashed lines represent the posterior boundary of Foxg1 domain in Foxd1^+/+ embryos. Note that in the most lateral sections (level 1) of Foxd1 deficient embryos, the Foxg1 expression domain is expanded posteriorly behind the optic recess, the Zic2 domain is reduced, and Islet1 appears to be normal. In more medial sections (level 2), the Foxg1 expression domain is expanded caudally, there is no Zic2 expression, and Islet1 expression is restricted to the most posterior aspect of the chiasmatic region. Bottom panels, schematics comparing the expression patterns of Foxd1, Foxg1, Zic2 and Islet1 in Foxd1^+/+ (left) and Foxd1^lacZ/lacZ (right) embryos, as viewed in sagittal sections. OC, optic chiasm; OR, optic recess; SOA, supraoptic area; HYP, hypothalamus; III, third ventricle. Scale bars: 200 µm. (B) Top left panel is a lateral view of an E15.5 embryo head, and the red-dashed line indicates the plane and level of section showed in panels a-d. Top right panel is a drawing of a frontal section; red-dashed square indicates the area shown in panels a-d. (a,b) In situ hybridization for Foxg1 in frontal cryosections of E15.5 Foxd1^lacZ/lacZ (b) and Foxd1^+/+ (a) embryos. (c,d) Immunostaining against Zic2 (green) in the same sections as in a and b, respectively. Bottom panels are summary cartoons comparing the expression patterns of Foxd1, Foxg1, Zic2 and Islet1 in Foxd1^+/+ (left) and Foxd1^lacZ/lacZ (right) embryos, in frontal view. Note that Foxg1 expression is expanded ventrally and Zic2 expression is restricted to a few cells in the most lateral aspect of the chiasm; however, Zic2 expression is normal more dorsally. Scale bars: 200 µm.

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