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Fig. 1. Pleiotropic phenotypes of ncr-2; ncr-1 mutants. (A-C)
Characteristic morphology of ncr-2; ncr-1 dauer-like larvae. (A)
ncr-2(nr2023); ncr-1(nr2022) dauer-like larva. (B)
daf-9(m540) dauer-like larva. (C) daf-7(e1372) dauer larva.
Left and right panels show the pharynx and the alae, respectively. In the left
panels, the black and white arrowheads indicate the isthmus and the terminal
bulb of the pharynx. Compared with the fully constricted pharynx of a
daf-7 dauer, which is similar to normal dauers, those of the
ncr-2; ncr-1 and daf-9 mutants are only partially
constricted. In the right panels, the black arrowheads indicate the dauer
lateral alae consisting of morphologically distinct longitudinal ridges. (D-G)
Pleiotropic developmental phenotypes of ncr-2; ncr-1 adults. (D) In a
young wild-type adult, normal alae consist of three or four parallel
longitudinal ridges. (E) Broken alae in an ncr-2; ncr-1 adult. White
arrowheads indicate the remaining faint alae up to the point where they are
broken. (F) Normal vulval morphology of a wild-type adult. The vulva is a
slightly protruding structure, forming the opening through which fertilized
eggs are expelled from the uterus. (G) Abnormal vulval morphology of a
ncr-2; ncr-1 mutant. The white arrow indicates the vulval protrusion
and black arrowheads indicate late stage embryos about to hatch inside the
parent. Scale bars: 20 µm.
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