spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Progesterone treatment of wild-type worms mimics phenotypes caused by cholesterol deprivation. (A,C) Normaski micrographs of wild-type worms grown on normal plates. (B,D) Normaski micrographs of representative wild-type worms cultured on low-sterol NGM plates containing 20 µg/ml progesterone. The phenotypes of Gen 1 adults are presented (see Materials and methods). (A) Normal gonadal cell migration. (B) Abnormal gonadal cell migration in animals cultured on progesterone plates. The distal tip cell migrated on the ventral side all the way up to the pharynx, failing to make either of the programmed turns. The black arrow indicates the direction of dtc migration. (C) Under normal conditions, the sperm (white arrowheads) are enclosed within spermatheca (sp). (D) Loose sperm in N2 animals cultured on progesterone plates. White arrowheads indicate sperm found within pseudocoelomic space, probably owing to rupture of the basement membrane surrounding the somatic gonad. Scale bars: 20 µm.





Right arrow Return to article