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Fig. 4. ncr-1 and ncr-2; ncr-1 mutants are hypersensitive to
progesterone. Synchronized populations of N2, ncr-1, ncr-2, or
ncr-2; ncr-1 strains (Gen 1, see Materials and methods) were cultured
on NGM plates with (+) or without (–) 5 µg/ml cholesterol and 20
µg/ml progesterone. The development of wild-type and mutant worms were
scored after 72 hours at 20°C. The results from duplicate experiments were
pooled together and expressed as a percent value (n>50).
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