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Fig. 10. Multiple Notch receptors regulate zebrafish intrahepatic biliary development. (A-I) Confocal projections through the liver of 5-dpf zebrafish larvae processed for cytokeratin IHC. (A) Wild-type intrahepatic biliary ducts. (B) Normal biliary development in notch 2 morphants. (C) Rare hepatocyte rosettes are seen in notch 5 morphants (arrow). (D) notch 2/5 morphants have rosettes (arrow) and reduced duct density. (E,F) Severe biliary defects in jagged 2/notch 2 and jagged 2/notch 5 morphants. Note rosettes (arrows) and also pronounced vascular cytokeratin staining in the jagged 2/notch 2 morphant. (G) jagged 3 knockdown does not augment the mild notch 5 morphant biliary phenotype (arrow, rosette). (H,I) 96-hpf hsp70:GAL4 and hsp70:GAL4; UAS:notch1aICD transgenic larvae processed for cytokeratin IHC. Ectopic biliary ducts (arrows) are only visible in the bigenic larvae following heat shock at 74 hpf and 86 hpf. Asterisk indicates endothelial cytokeratin staining.

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