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Fig. 3. Zebrafish intrahepatic biliary development. (A-C) Tissue cross-sections of
60-hpf (A) and 70-hpf (B) embryos and a 80-hpf larva (C) processed for
cytokeratin IHC. Nascent ducts (arrows) within the developing liver are
evident at 60 hpf (A) and 70 hpf (B). By 80 hpf (C), a branching ductular
network (arrows) is evident. Arrowheads in A,B indicate the origin of the
extrahepatic duct. (D) Confocal projection of intrahepatic bile ducts in a
5-dpf larva processed for cytokeratin IHC. (E) Tissue section of a 70-hpf
larva processed for P-glycoprotein IHC. Developing canaliculi (arrowheads) are
evident between adjacent hepatocytes. (F) Confocal projection of hepatocyte
canaliculi within the liver of a 5-dpf larva – note the elongated,
tubular canalicular structure. (G,H) Confocal projection generated from
contiguous Z-sections of a 75-hpf larva (G) and a 5-dpf larva (H) processed
for cytokeratin IHC. Intrahepatic bile ducts emerge from the liver to form the
common hepatic duct (chd), and join the cystic duct (cd) and common bile duct
(cbd) that inserts into the intestine (i). (I) Transmission electron
micrograph of a 70-hpf larva, showing a developing canaliculus (c) near the
hepatocyte nucleus (hn). (J) Canaliculus of a 70-hpf larva anastomosing with a
bile duct composed of two ductular cells (dc). (K,L) Transmission electron
micrograph from a 5-dpf larva, showing a ductular cell within a hepatocyte
tubule anastomosing with several canaliculi from surrounding hepatocytes (K).
(L) Distal portion of a bile duct within the center of a hepatocyte tubule.
Electron-dense particles within bile are evident in the duct lumen. e,
esophagus; g, gall bladder; l, liver; pa, pancreas; p, pronephric duct.
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