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Fig. 6. jagged gene knockdowns perturb craniofacial and cardiac development. (A-E,G) Lateral views of (A) wild type, and (B) jagged 1, (C) jagged 2, (D) jagged 3 and (E,G) jagged 2/3 morphants. Note, forebrain and midbrain defects in jagged 1 morphants (arrowheads) and mild craniofacial defects in the jagged 3 (D) and jagged 2/3 (E,G) morphants. All morphants, except jagged 2, have small ears (arrows). jagged 2/3 morphants have pericardial edema (open arrowhead). (F,H) Alcian Blue staining of 5-dpf wild-type (F) and jagged 2/3 morphant (H) larvae, lateral view. These stainings show that the ethmoid (e), palatoquadrate (pq) and ceratohyal (ch) cartilages of jagged 2/3 morphants are smaller than in wild-type siblings. Similar findings are observed in jagged 3 morphants (not shown). cb, ceratobranchial; hs, hyosymplectic; m, Meckel's cartilage; oa, occipital arch.





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