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Fig. 3. Generation and characterization of the Df(2R)ED3716^CG2 Fak56 deletion mutant. (A) Genetic analysis of Fak56. Upper panel: the Fak56 locus is depicted together with the surrounding genes (as before, Fig. 1). The two P-element insertions used to generate Df(2R)ED3716^CG2, P(5-SZ-3124) and P(UM-8250-3) are shown by inverted triangles. Lower panel: FRT recombination between the 5-SZ-3124 and UM-8250-3 P elements resulted in a 12.8 kb deletion, named Df(2R)ED3716^CG2, which uncovers both the entire Fak56 ORF as well as the neighboring Calpain A gene. The Fak56 genomic rescue fragment is also indicated (striped box). (B) Mapping of the breakpoints in the Df(2R)ED3716^CG2 chromosome. The sequence flanking Df(2R)ED3716^CG2 is shown. (C) Southern analysis of the Df(2R)ED3716^CG2 mutant. The entire ORF of the Fak56 gene is absent in Df(2R)ED3716^CG2. Genomic DNA from (i) Df(2R)ED3716^CG2/Fak56 ^CG1, (ii) Df(2R)ED3716^CG2/+, and (3) wild-type flies were probed with FAKI and FAKII (covering 5' and 3' regions of the Fak56 gene respectively) to show that the Fak56 ORF is completely absent in Df(2R)ED3716^CG2 animals (^* indicates the RFLP generated from the Fak56^CG1 mutant).

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