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Fig. 6. Overexpression of Fak56 results in embryonic muscle detachment. Wild-type
embryos and embryos expressing Fak56 specifically in embryonic muscle, using
the Mef2-GAL4 driver, were stained with anti-ßPS antibodies (green) to
visualize integrins and Phalloidin (red) to visualize actin. Embryos
overexpressing Fak56 specifically in embryonic muscle, show a potent muscle
detachment phenotype (E,F, arrows), when compared with wild-type (B,C).
Interestingly, in spite of obvious rounding up of somatic muscles, integrins,
as monitored by anti-ßPS-integrin antibodies, were still localized at the
ends of the muscles that remain attached in Fak56 overexpressing embryos (D,
arrowhead), indistinguishable to wild-type embryos (A, arrowhead).