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Fig. 2. lqf promotes dorsal muscle development. Dorsal views of stage 15 wild-type (A,B) and lqfAG embryos (C,D) stained for myosin heavy chain (MHC; A,C) to label the heart and somatic muscles and Eve (B,D) to label nuclei within the DA1 muscle and Eve-pericardial cells (EPC). (A) MHC expression outlines the muscle pattern in the dorsal mesoderm. (C) In lqfAG embryos, the muscle pattern appears grossly normal but the size of dorsal muscles appears reduced. To aid visualization of muscles, lines demarcate the size and orientation of the DA1 and DO2 dorsal muscles and arrowheads identify the heart (A,C). (B) In wild-type embryos, each DA1 muscle (dashed circles) contains roughly 11 Eve-positive nuclei while DA1 muscles in lqf embryos (D; dashed circles) contain on average eight Eve-positive nuclei. Arrowheads in B and D identify EPC. Anterior – left.





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