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Fig. 6. Ce-epn-1(RNAi) enhances the premature meiotic entry defect of glp-1(bn18) mutants. (A) Dissected gonad arms stained with DAPI to visualize DNA morphology. Distal is left and the proximal portions of the gonad arms are not shown. Brackets mark the regions of gonad arms containing proliferative nuclei. Wild-type (top), Ce-epn-1 (RNAi) (second from top) and glp-1(bn18) (third from top) animals all contain a proliferative zone one day past the fourth larval stage, however the proliferative zones are smaller in the epn-1(RNAi) and glp-1(bn18) animals. glp-1(bn18); epn-1(RNAi) animals (bottom) of the same age lack a proliferative zone, but rather have sperm extending to the distal end. All animals were grown at 20°C. Scale bar: 20 µm. (B) Quantification of percentages of worms that exhibit strong glp-1 phenotype, lack of proliferative zone phenotype. ^aGonad arms were determined to be Glp if they had sperm or oocytes at the very distal end of the gonad or if no proliferative cells were present as evidenced by HIM-3(plus)/REC-8(minus) cells at the very distal end of the gonad. ^bN refers to the number of gonad arms examined. ^cThis percentage of Glp is somewhat higher than what has previously been described for glp-1(bn18) at 20°C, however this is probably due to the plates being slightly above 20°C prior to placing the eggs on them. (C) Quantification of proliferative zone size in worms that contain reduced proliferative zone based on HIM-3 and REC-8 staining. Error bars represent s.d.

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