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Fig. 1. Mutant embryos develop to blastocysts but exhibit a phenotype at early
gastrulation. Immunohistochemical detection of ß-catenin in wild-type (A)
and mutant (B) ovaries revealed enhanced cytoplasmic localization of
ß-catenin in mutant oocytes. (C) Genotyping of single oocytes from
ß-catEx3flox/+;cre/+females by PCR demonstrates the efficient
deletion of the floxed exon 3 of the ß-catenin gene by the
oocyte-specific cre-recombinase; tail DNA from the same females was used as
the control. (D) Western blot of cell lysates from mutant and wild-type (R1)
ES cells shows the expression of the stabilized form of ß-catenin in the
mutant cells. (E-H) Immunofluorescent detection of ß-catenin in wild-type
(E), and mutant (F) blastocysts, and simultaneous detection for E-cadherin
(green) and Oct4 (red) (G, wild type; H, mutant) revealed no differences
between these embryos. (I-J) Phenotype of mutant (J,K) and wild-type (I) E6.5
embryos revealed that the epiblast of mutant embryos varied, being small or
disorganized. Scale bar: 50 µm.
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