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Fig. 5. Premature epithelial-mesenchymal transition in the epiblast of mutant embryos. Immunohistochemical analysis of E6.5 mutant embryo serial sections showed that some cells of the embryonic ectoderm (arrow) that were negative for E-cadherin (A) expressed T-Brachyury (B) and Lef1 (C), demonstrating their change from an epithelial to a mesenchymal cell fate. Arrow indicates cells that changed cell fate. Scale bar: 100 µm.





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