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Fig. 6. Embryonic patterning is changed as a result of stabilized ß-catenin expression. The expression of embryonic patterning genes was analyzed by whole-mount in situ hybridization of E6.5 wild-type and mutant embryos. In wild-type and mutant embryos Bmp4 had a similar expression pattern in the extra-embryonic ectoderm. The expression domain of Otx2 was less extensive in the mutant embryo epiblast in comparison to that of the wild-type embryo. No Nanog expression was found in the mutant epiblast. The mutant visceral endoderm (arrow) expressed no Hex. Arrow indicates mutant visceral endoderm negative for Hex; EE, embryonic ectoderm.





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