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Fig. 7. Mutant embryos exhibit a restricted differentiation potential. (A,B)
Determination of ß-galactosidase expression in sections from E7.5 embryos
that developed from aggregation chimeras between ROSA26 and mutant morulae
revealed that mutant cells contributed mostly to extra-embryonic tissues and
were only occasionally found in the embryonic ectoderm of the chimeras. In
some cases the embryonic ectoderm cell layer was exclusively composed of
ROSA26 cells (A,B). (C-E) Analysis of wild-type and mutant E6.5 embryos
transplanted under the kidney capsule to induce teratomas. (C) Teratocarcinoma
produced by transplantation of wild-type embryo. Several well differentiated
tissues are visible, including bone, muscle, neural tissue and skin
epithelium. (D) Adjacent section stained with antibody to ß-catenin.
Higher magnification of area marked in panel C shows expression of
ß-catenin in the membranes of the epithelial cells. (E) Histological
section of tumor derived from transplanted mutant embryo. Cells similar to
those present in extra-embryonic endoderm form small glandular structures
(arrowhead). Hyalin substance corresponding to Reichert's membrane (arrow)
fills extracellular spaces. Certain gland-like structures display substantial
cellular and nuclear polymorphism (star). (F) Adjacent section stained with
antibody to ß-catenin. There is intensive membrane and cytoplasmic
staining. Cells exhibiting polymorphism (star) also show nuclear staining.
Scale bar: 100 µm.
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