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Fig. 1. Low-level tsg1 knockdown causes a ventral tail vein edema and cell
death in anterior structures, but does not alter patterning during early
somitogenesis. Uninjected embryo (A); a wild-type embryo injected with 8 ng
MO1 (B) displays a slightly reduced head and a large edema in, or dilation of,
the ventral tail vein (arrowhead), in which the blood accumulates and
circulation slows. (C) The vein edema can be rescued by injection of
tsg1 mRNA (10% with edema phenotype, n=133, not shown) or
tsg2 mRNA (8% with edema phenotype, n=106), whereas the
reduction in head size is unaltered. Scale bars in A-C show the diameter of
the eye as a measure of the extent of anterior tissues. TUNEL analysis as a
measure of cell death in 24 hours post fertilization (hpf) uninjected
wild-type (D) and 8 ng MO1-injected sibling (E) embryos. Cell death is not
rescued by co-injection of tsg mRNA (F). (D-F) Anterior towards the
left, dorsal view posterior to the MHB boundary. (G-N) Whole-mount in situ
hybridization of uninjected embryos (G-J) or embryos injected with 8 ng MO1
(K-N). (G,K) Expression of pax2.1 (arrowhead) and krox20
(bracket) in the mid/hindbrain (MHB) boundary and rhombomeres 3 and 5,
respectively. (H,L) More posterior views of the embryos in G and K.
pax2.1 expression in the pronephric system (arrowheads) and
myoD in anterior somitic mesoderm is unchanged. (I,M) Lateral views
of untreated or injected embryos showing no alterations in anterior expression
of pax2.1 in the eye field (asterisk). (J,N) Expression of
bmp4 anteriorly (asterisk) and in the tail bud (arrowhead) at the
three-somite stage is normal in injected embryos. Insets show tail bud views.
(G-I,K-M) Eight somites. (J,N) Three somites. (G,H,K,L) Dorsal views with
anterior upwards. (I,J,M,N) Lateral views, anterior is upwards.
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