|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Knockdown of Tsg function partially suppresses the chordino
ventralization. (A) Uninjected chordino homozygote; arrowheads
indicate multiple ventral fin folds, also seen in a slightly different view at
higher magnification (inset). Appearance of multiple fin folds is suppressed
by injection of 8 ng MO1 (B, inset shows dorsal view of tail of same embryo).
(C-H) Injection of 25 ng MO5 into chordino homozygotes results in a
substantial enlargement of dorsally derived tissues. Examination of live
embryos at the six-somite stage showed reduced head neural tissue in C and
suppression of the reduction in F (arrowheads). (C) Uninjected
chordino homozygote; (F) injected chordino homozygote;
lateral views, anterior is upwards. In situ analysis at the same stage with
pax2.1 (arrowhead), krox20 (bracket) (D,G) and myod
(E,H) showed that the somites, the MHB and rhombomeres 3 and 5 are increased
in size in the injected (n=14/20) compared with uninjected mutants.
(D,E) Uninjected mutants; (G,H) two views of the same injected mutant. In the
injected mutant, the anterior neural and somitic mesoderm appears similar to
wild type; however, the tail bud is still enlarged, indicating that the
dino phenotype is not fully suppressed.
|
