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Fig. 1. Tagged nos and nos-tub3'UTR transgenes.
(A) The gfp-nos and gfp-nos-tub3'UTR
transgenes contain GFP sequences (dark shading) inserted at the N terminus of
the genomic nos coding region (light shading). Both transgenes
include the nos promoter and 5' regulatory sequences (Pnos),
5'UTR (left black bar) and polyadenylation signal. Gfp-nos
(top) bears the intact nos 3'UTR (right black bar), whereas
these sequences have been replaced by 
-tubulin 3'UTR
sequences (tub) in gfp-nos-tub3'UTR (bottom). (B) The
hemagglutinin (HA) epitope tagged nos-tub3'UTR
transgene is identical to gfp-nos-tub3'UTR, except
that an N-terminal HA tag replaces GFP. The nos-tub:TCE and TCE
mutant transgenes carry insertions of wild-type and mutant TCE sequences
(shown in C), respectively (hatched box). Transgenes in A and B are drawn to
scale, except for introns. (C) Nucleotide changes associated with the
SRE– (circles), TCEIIA (squares) and TCEIIIA (hexagons)
mutations are indicated on the TCE secondary structure. The following
mutations are not shown: TCEIIIA/U^C72, a compensatory mutation to TCEIIIA
that restores base pairing with three A to U substitutions; and TCEIIIGC/GC,
which changes the alternating U-A and A-U base pairs in the distal region of
stem-loop III to alternating G-C and G-C base pairs
(Crucs et al., 2000).
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