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Fig. 3. GFP-Nos distribution at late stages of oogenesis. (A-C) Lower power images
of the anterior region of fixed 6 x gfp-nos egg
chambers used for staging. (D-F) Higher power images of the posterior region
of egg chambers shown in A-C. (A,D) Stage 11 egg chamber, with transfer of
GFP-Nos produced in the nurse cells to the oocyte during nurse cell dumping.
(B,E) Stage 12 oocyte, with GFP-Nos distributed uniformly throughout the
ooplasm. (C,F) Stage 13 oocyte, in which GFP-Nos has disappeared from the
anterior and a gradient of GFP-Nos is observed emanating from the posterior
pole. This gradient is also detected in 2 x gfp-nos
oocytes (see Fig. S1 in the supplementary material). The actin cytoskeleton is
labeled with rhodamine-phalloidin (red) in all panels.
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