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Fig. 2. Normal cleavage pattern of the O/P lineage. (A) Image of the o (red) and p
(green) bandlets in the germinal band of a stage 8 embryo. Ventral is towards
the left, and anterior towards the top. Nuclei are stained with Hoechst 33258,
and appear blue. (B) Hoechst staining shows the shape and location of the
nucleus of each individual cell. One o primary blast cell is undergoing
mitosis (red arrowhead), and the more anterior (i.e. older) o blast cell
clones consist of the larger o.a daughter cell and the smaller o.p daughter
cell (arrows). Further anterior, an o.a cell has divided into two cells, o.aa
and o.ap. The most posterior cells in the p bandlet are primary blast cells.
Anterior to the p primary blast cells, the older p blast cell clones consist
of paired daughter cells p.a and p.p. A p.a cell undergoing mitosis is labeled
with a yellow arrowhead. Scale bar: 10 µm.
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