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Fig. 4. Effects of the bilateral ablation of the Q lineage on the cleavage patterns
of o/p blast cell clones in stage 8 embryos. (A,B) Cleavage pattern of the
presumptive o bandlet (A) and the presumptive p bandlet (B) of H.
robusta (Austin) shown by Hoechst 33258 nuclear staining. Presumptive o
blast cell clones express the normal O-type cleavage pattern. The o.p nuclei
are marked with red arrows. Most presumptive p blast cell clones express a
normal P-type cleavage pattern, and white lines mark the p.a and p.p daughter
cell pairs. A few presumptive p blast cell clones express an O-type cleavage
pattern (yellow arrows). (C,D) Ablation of bilateral Q lineages causes both
o/p bandlets to express the O-type cleavage pattern in H. robusta
(Sacramento) (C) and Helobdella sp. (Galt) (D). The small nuclei of
o.p cells in the presumptive o bandlet are labeled with red arrows, and the
nuclei of ectopic o.p cells in the presumptive p bandlet are labeled with
yellow arrows. See Fig. 2 for
orientation. Scale bar: 20 µm.
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