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Fig. 3. (A) Expression of bmp4 in the LNE on one side of embryo (right)
reduced six1 placode expression compared with control, uninjected
side (left). (B) Ventral epidermis containing chordin-expressing
(red) cells in a dispersed pattern; there is no ectopic six1
expression. (C) Ventral epidermis containing a secondary axis (sox2,
blue) after ectopic chordin expression (red). (D) Ventral epidermis
containing a secondary axis/elongated clone (*) after ectopic
chordin expression (red); ectopic six1 expression is at its
anterior pole (stripe between arrows). (E) When co-injection of
frzb-1+noggin mRNAs does not form a secondary axis
(dispersed red cells), ectopic six1 is not induced. (F) When
co-injection of frzb-1+noggin mRNAs forms a secondary axis
(left), the ectopic six1 domain (arrow) extends further posterior
(black bar) from the anterior tip of the secondary axis (*),
compared with noggin alone embryos (right). (G) Co-injection of
dnWnt8+noggin mRNAs expands the six1 expression
domain (arrows) to encircle both the primary axis (*) and the
induced secondary axis (red cells, inset). (H) Wnt8 expression in the LNE
(left side) represses six1 (arrow). (I) cFGFR1 expression in the LNE
(left side) represses six1 (arrow). (J) Explants were injected with
either cfgfr1 or Wnt8 mRNA and cultured in 1 ng/ml Noggin.
The high levels of six1 expression induced by this concentration of
Noggin were significantly repressed by both factors. (K) Expression of
frzb-1+noggin mRNAs either represses (left) or reduces
(right) foxD3 expression on the treated side (arrows). (L) Wnt8
expression in the LNE (left side) expands foxD3 (arrow). (M) cFGFR1
expression in the LNE (left side) expands foxD3 (arrow).
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