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Fig. 4. Examination of differentiation markers in
Mmp13–/– endochondral cartilage. (A) BrdU
incorporation in 1-week-old tibia of wild-type and
Mmp13–/– mice. Animals were injected with 1
µg BrdU/g mouse 1 hour prior to sacrifice. A similar number of
BrdU-positive cells (brown staining) can be observed in the proliferating
chondrocyte (PC) zone, and in cells within the trabecular bone (TB) for
wild-type and Mmp13–/– bones, indicating that
proliferation is not impaired in Mmp13–/–
mice. (B) In situ hybridization on 1-week-old metatarsals with probes to
chondrocyte differentiation markers show normal expression domain of col2, but
expanded expression of collagen type X and VEGF, indicating an increased late
hypertrophic chondrocyte population in the
Mmp13–/– growth plate. In wild-type mice,
expression of osteopontin (Osp) is restricted to only one row of
hypertrophic chondrocytes in the growth plate of wild-type mice. The growth
plate of Mmp13 –/– mice contains several rows
of chondrocytes expressing Osp (broken line demarcates front of
ossification). Safranin-O staining shows general morphology (HC, hypertrophic
cartilage). Scale bars: 100 µm.
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