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Fig. 9. Altered ECM remodeling in Mmp9–/–,
Mmp13–/– and
Mmp9–/–;Mmp13–/–
long bones. (A) Immunostaining of 2-week-old tibia of wild-type,
Mmp9–/–,
Mmp13–/– and
Mmp9–/–;Mmp13–/–
mice with an antibody to the DIPEN neoepitope of Mmp-cleaved
aggrecan. Cleaved aggrecan (brown staining) is present along the primary front
of ossification in wild-type, Mmp9–/– and
Mmp13–/– bones, no positive staining is
apparent in
Mmp9–/–;Mmp13–/–
bones. (B) Immunostaining of 2-week-old tibia of wild-type,
Mmp9–/–,
Mmp13–/– and
Mmp9–/–;Mmp13–/–
mice with an antibody to the 3/4 cleavage fragment of col1 and col2. The
primary front of ossification (indicated by a broken line) shows the presence
of cleaved collagen (pink staining) in transverse septa surrounding the most
terminal hypertrophic chondrocytes (arrows) in wild-type and
Mmp9–/– bones, along the trabeculae of the
developing trabecular bone (arrowheads) in wild-type and
Mmp13–/– bones, and at random throughout the
hypertrophic chondrocyte zone (arrows) and trabecular bone (arrowheads) in
Mmp9–/–;Mmp13–/–
bones. Scale bars: 200 µm in A; 100 µm in B.
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