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Fig. 3. Par-1, Exu-GFP and bicoid mRNA localise to patches in the nurse cells. (A-C) Confocal section in the apical region of the nurse cells of egg chambers expressing Exu-GFP. (A) Par-1 protein is visualised with an anti-Par-1 antibody (red) and Exu-GFP with an antibody against GFP in the wild type. Both proteins accumulate in patches and colocalise almost throughout the whole nurse cell cytoplasm. Par-1 is also detected at the membranes of the nurse cells. (B) Double staining for Exu-GFP (green) detected with antibody against GFP and bicoid mRNA (red) in the wild type. (C) exu null mutants (exu^XL/exu^VL) expressing Exu-GFP protein in which all phosphorylation sites are mutated (Exu-GFP^A+B) double stained for GFP (green) and bicoid mRNA (red). (D,E) Confocal sections through the middle of a wild type egg chamber expressing wild-type Exu-GFP (D), and an exu null mutant egg chamber expressing Exu-GFP^A+B (E). Exu-GFPs are visualised with fluorescence of the GFP.

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