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Fig. 8. exu independent functions of par-1 in bicoid mRNA localisation. In situ hybridisation showing bicoid mRNA localisation in confocal sections of stage 9 egg chambers, in which in the wild-type mRNA is localised as a ring around the anterior margin of the oocyte. (A,B) exu-null mutant egg chambers either expressing no transgene (A) or a transgene encoding the activated Exu^Ser457Glu (B). Both egg chambers are from siblings from the same cross and have been stained in parallel. The Exu^Ser457Glu encoding transgene rescues bicoid mRNA localisation completely, as revealed by the restriction of bicoid mRNA to the anterior corners of the oocyte. The anterior bicoid mRNA ring appears as two discrete dots in the confocal section. (C,D) Egg chambers from par-1 mutant siblings either expressing no transgene (C) or the Exu^Ser457Glu encoding transgene (D). Both egg chambers have been stained in parallel. The Exu^Ser457Glu protein is unable to fully rescue the cortical localisation of bicoid mRNA as the mRNA is no longer tightly restricted to the anterior corners but spreads along the lateral and anterior cortex of the oocyte (D). (E,F) For clarity only the oocyte of the egg chamber is shown. bicoid mRNA is restricted to the anterior corners in the wild type (E) and is dispersed throughout the ooplasm in homozygous exu^PJ mutants (F). In homozygous par-1^9A exu^PJ double mutants (G), bicoid mRNA is cortically localised resembling the situation in par-1 single mutants.

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