|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. RT-PCR and in situ hybridization analysis of zebrafish dpr1 and
dpr2. (A,B) RT-PCR analysis (25 embryos per stage) of dpr1
(A) and dpr2 (B). Zebrafish max is used as a
loading/positive control. –RT is a control for genomic DNA
contamination. (C,D) In situ hybridization for expression of dpr1 (C,
parts a-f) and dpr2 (D, parts a-f), with embryos shown that are
representative of at least 25 analyzed per stage. Stages: (a) sphere stage;
(b) shield stage; (c) 80% epiboly; (d) 
tailbud; (e) five somites (in C),
three somites (in D); (f) 12 somites. Orientation: (a,b) animal views,
dorsal is rightwards; (c,d) dorsal views, anterior is upwards; (e,f) anterior
is leftwards. (C, part b) Arrow indicates margin and arrowhead indicates
shield. (C, part d) dpr1 (blue), pax2.1 (red,
midbrain/hindbrain boundary) and krox20 (red, –r3 and 5). Black
arrow indicates dpr1 expression in brain. Black arrowhead indicates
dpr1 expression in anterior spinal cord. Red arrow indicates
pax2.1. Red asterisk indicates krox20. (C, part e) Black
arrow is posterior limit of dpr1(blue) expression. Red arrow is
posterior limit of pax6.1(red) expression. Arrowheads indicate r3 and
r5. (C, part f) Arrow indicates anterior older somite. Arrowhead indicates
staining of posterior, younger somite. Asterisk indicates presomitic mesoderm.
Yellow arrow indicates tailbud. (D, part b) Arrow indicates margin and
arrowhead indicates shield. (D, part d) Brackets indicate mediolateral banding
pattern of staining in lateral mesoderm. (D, part e) Arrow indicates boundary
of krox20 (red) and dpr2 (blue) staining. (D, part f) Arrow
indicates staining in the anterior of the older somites. Arrowhead indicates
presomitic mesoderm.
|
