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Fig. 8. Dpr synergy requires CK1 ${epsilon}$ or CK2. (A) Dvl2 or ß-catenin activation of canonical target genes does not require CK1 ${epsilon}$ , but Dvl2-CK2 synergy does require CK1 ${epsilon}$ . Lanes 1 and 2, 0.4 ng of dvl2, 1 ng of ck2 ${alpha}$ and 1 ng of ck2ß RNA; lanes 3 and 4, 0.8 ng dvl2 RNA; lanes 5 and 6, 0.1 ng stabilized ß-catenin RNA. (B) Dpr1-Dvl2 and Dpr1-Par1 synergy requires CK1 ${epsilon}$ , but Dpr1-CK2 synergy does not. The amounts of RNA injected were 1 ng of dpr1, 1 ng of par1, 0.4 ng of dvl2, 1 ng of ck2 ${alpha}$ and1 ng of ck2ß. CKI-7 (5 nl of 2.5 mM) was co-injected in experiments in A and B. (C) Table of Dpr1 synergistic interactions and requirement on CK1 ${epsilon}$ . Asterisk indicates higher doses of Dvl2 RNA that could activate alone did not require CK1 ${epsilon}$ , but Dvl2-CK2 synergy did require CK1 ${epsilon}$ .

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