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Fig. 5. Robo, Robo2 and Robo3 are expressed in overlapping patterns in the visual
system. (A,D,G) Third instar nervous systems stained with antisera against
indicated Robo family member. (B,E,H) Lateral view of optic lobe stained with
antisera against indicated Robo family member (magenta), neuronal nuclei
stained with anti-Elav (blue), and photoreceptor axons visualized with GMR:GFP
(green). (C,F,I) Robo family staining alone. (A) Robo is expressed in the
developing optic lobes (arrowheads). (B,C) Robo is expressed by IPC
neuroblasts, by distal cell neurons, and in the medulla cortex. (D) Robo2 is
expressed in the developing optic lobes (arrowheads). (E,F) Robo2 is expressed
by IPC neuroblasts and distal cell neurons. (G) Robo3 is expressed in the
developing optic lobes (arrowheads). (H,I) Robo3 expression is not detected in
IPC neuroblasts, but is detected in distal cell neurons and in the medulla
cortex as well as in photoreceptor axons. IPC, inner proliferation center;
lamn, lamina neuropil; medc, medulla cortex.
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