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Fig. 7. Distal cell neurons intermingle with lamina glia in Robo family knockdowns.
(A,B) Lateral view (anterior at bottom). IPC neuroblasts and distal cell
neurons are visualized with anti-Fas3 (red), c155:GFP is most strongly
observed in IPC neuroblasts and photoreceptors (green), and neuronal nuclei
are visualized with anti-Elav (blue). (A) c155-Gal4;UAS-GFP. (B)
Distal cell neurons enter the lamina in
c155-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animals
(arrow), reaching anterior edge of lamina (arrowhead). (C-F) Animals express
GFP (green) under control of Sca-Gal4, labeling the IPC, distal cell
neurons and medulla cortex. Neuronal nuclei are visualized with anti-Elav
(blue). (C,D) Photoreceptor axons are visualized using anti-Chaoptin (red).
(C) Sca-Gal4;UAS-GFP animal. (D) Distal cell neurons enter the lamina
in Sca-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animals
(arrow). (E,F) Lamina glia are visualized using anti-Repo (red). Distal cell
neurons intermingle (arrows) with lamina glia in Robo family knockdown
animals. (E)
Sca-Gal4;UAS-GFP;UAS-RoboRNAi,UAS-Robo3RNAi;UAS-RoboRNAi,UAS-Robo3RNAi
animal. (While Sca-Gal4;UAS-RoboRNAi;UAS-Robo3RNAi animals had no
defects, animals containing two copies of both UAS-RoboRNAi and
UAS-Robo3RNAi had modest defects, consistent with overlapping roles
of Robo family members.) (F)
Sca-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animal.
dcn, distal cell neuron progeny; IPC, inner proliferation center; lam, lamina;
medc, medulla cortex.
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