spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 8. Robo family knockdown disrupts photoreceptor axon targeting. (A-C) Photoreceptor axons visualized using anti-Chaoptin. (A) Tubulin-Gal4;UAS-GFP control. (B) Tubulin-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animal, showing many photoreceptor axons extending through the lamina (arrow) and too many photoreceptor axons entering the medulla (arrowhead). (C) GMR-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animal. (D-F) Animals express GFP (green) under control of Sca-Gal4, while photoreceptor axons are visualized using anti-Chaoptin (magenta). (D) Sca-Gal4;UAS-GFP animal. (E) Sca-Gal4;UAS-GFP;UAS-RoboRNAi,UAS-Robo3RNAi;UAS-RoboRNAi,UAS-Robo3RNAi animal in which GFP-expressing cells in the lamina correspond to regions of photoreceptor axon mistargeting (arrow). (F) Sca-Gal4;UAS-GFP;UAS-RoboRNAi;UAS-Robo2RNAi;UAS-Robo3RNAi animal. (G) Schematic of observed disruptions in visual system development. In wild type, distal cell neurons (blue) express Robos (orange outline), while Slit protein (red) surrounds glia (yellow with black outline) at the base of the lamina. Lamina glia serve as initial targets of incoming R1-R6 photoreceptor axons (green). When expression of all three Robo family members is inhibited in distal cell neurons (robo, robo2, robo3), distal cell neurons intermingle with the lamina glia and photoreceptor axon targeting is disrupted. Loss of Slit expression (slit) causes an indistinguishable defect.





Right arrow Return to article