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Fig. 1. Phenotype of maternally rescued mau-2 animals. (A) Detail of the
dorsal cord and the vulval muscles in young adult worms carrying the reporter
unc-31::gfp used to visualize the nervous system and the vulval
muscles. The dorsal cord is normal in the wild type (a), defasciculated in
mau-2(qm5) mutants as indicated by the arrow (b), and normal in
maternally rescued mau-2(qm5) animals (c). The vm1 vulval muscles
(black asterisks) are normal in the wild type (e), and at least one of the vm1
is abnormally placed in mau-2(qm5) mutants and in maternally rescued
mau-2(qm5) animals, as indicated by the arrow (f). (B) Quantification
of the phenotype in maternally rescued mau-2(qm5) animals, compared
with the wild type and mau-2(qm5) mutants (n=50-200). Both
the placement of the soma and the projection of the axon of the AVM neurone
are completely maternally rescued. The axon of HSN could not be scored in
mau-2 mutants (red asterisk), as tph-1::gfp is not expressed
in HSNs for unknown reasons, while it is still expressed in the NSMs
(Sze et al., 2000).
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