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Fig. 3. The expression pattern of mau-2. (A) MAU-2 protein level in the wild type (N2) and in the four mau-2 mutant alleles. No MAU-2 protein is detected in the deletion and nonsense alleles, but a reduced level is detected in the mis-sense allele qm4 (arrowhead). A band is detected in transgenic worms carrying wild-type copies of the gene mau-2 [mau-2(qm160); qmEx[mau-2(+)]]. Total protein extract loaded per lane is 50 µg. (B) Northern analysis of mau-2 at all developmental stages (E, embryos; L1-L4, larval stages; A, young adults) and at the adult stage in mutant backgrounds. Total RNA extract loaded per lane is 10 µg. (C) Western analysis of MAU-2 at all developmental stages and at the adult stage in mutant backgrounds. Total protein extract loaded per lane is. (D) Western analysis of MAU-2 at three larval stages, comparing the MAU-2 level in the wild-type and maternally rescued worms. m⁺z⁺ worms were of the genotype dpy-5(e61) mau-2(+); m^–z^–, dpy-5(e61) mau-2(qm5) derived from a dpy-5(e61) mau-2(qm5) mutant hermaphrodites; and m⁺z^–, dpy-5(e61) mau-2(qm5) derived from dpy-5(e61) mau-2(qm5)/++ hermaphrodites. In each lane, 400 worms loaded. For the late L4 stage worms, the double band both for MAU-2 and tubulin is a migration artefact.

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