DEV01456 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Confocal microscope images of Tg[olig2:egfp] embryo obtained immediately after iontophorectic labelling. (A) Dorsal view, anterior towards the right, of z-image stack projection. Intersection of green and red lines marks single olig2:EGFP⁺ cell labeling with rhodamine dye. (B) Image from A rotated to present lateral view. (C) Image from A rotated to present transverse view. Scale bar: 10 mm.

• Supplemental Figure 2 -

  Fig. S2. Comparison of olig2 and Tg[olig2:egfp] expression. All panels are transverse views, dorsal upwards, of wild-type (wt) embryos hybridized with olig2 RNA probe (A,C) and Tg[olig2:egfp] embryos hybridized with egfp RNA probe (B,D). We are unable to examine the endogenous olig2 RNA expression pattern in transgenic embryos as the transgene produces olig2 RNA (Shin et al., 2003). In total, we have made nine independent transgenic lines (Shin et al., 2003) (data not published). The spatial expression pattern is identical for each and they differ only in the intensity of reporter protein fluorescence, which probably reflects different transgene copy number (Shin et al., 2003). Neural keel indicated by nc, spinal cord by sc. Scale bar: 20 mm.