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Fig. 3. Clonal analysis of olig2:EGFPD/L neural plate cells.
(A) Schematic representation of labeling strategy. (B) Dorsal view of
transgenic embryo immediately after labeling. (C-F) Side views, anterior
towards the left, dorsal upwards, of 2.5 dpf embryos showing examples of
labeled cells, which include SMNs, CiD interneurons, OPCs (asterisks) and
cells whose identities could not be determined (question marks). Arrowheads
mark some of the axon projections. (D') Combined images of EGFP and
rhodamine fluorescence of clone shown in D. The weak EGFP fluorescence of the
CiD interneuron is obscured by the bright rhodamine signal.
(D'',F') Schematic of clones shown in D,F. (G) Diagram, from a
dorsal view, summarizing fate mapping results showing cell fate bias on the
medial/ventral (m/v) to lateral/dorsal (l/d) axis of the neural plate/neural
tube. Broken line marks midline of neural plate. Scale bars: 20 µm.
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