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Fig. 6. Hh signaling is required for oligodendrocyte specification after
dorsoventral spinal cord patterning and motoneuron development. All panels in
the top five rows show transverse sections, dorsal upwards, of trunk spinal
cord. Bottom two panels are side views of whole embryos, dorsal upwards and
anterior leftwards. (A-E) Control embryos showing normal expression of various
markers. (G-K) Embryos treated with cyclopamine from 6 hpf onwards did not
express olig2 (G) or nkx2.2 (H), and expressed iro3
in ventral spinal cord (I), indicating that ventral spinal cord patterning was
lost. These embryos did not produce SMNs (J) or OPCs (K). (L-Q) Embryos
treated with cyclopamine from 30 hpf onward did not express olig2 by
36 hpf (L) but expressed nkx2.2 (M) and iro3 (N) in their
normal patterns. SMNs were produced in normal numbers (O) but OPCs were absent
(P,Q). (F) Untreated Tg[olig2:egfp] embryo showing OPCs (arrows) in
dorsal spinal cord (brackets). Scale bar: 20 µm for top five rows; 80 µm
for F and Q.
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