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Fig. 3. EMC expression pattern in ovaries. Anterior is to the left. (A,E) Females containing the emc^P5C enhancer trap, stained with anti-ß-galactosidase (red) and with anti-Fas3 (green), showing the germarium and a stage-2 egg chamber (A) or a stage-3 egg chamber and a polar/stalk group (E). Arrows in A indicate terminal filament, cap and inner sheath cells. The bracket in A indicates follicle cells in a germarium. The bracket in E indicates polar and stalk cells. (B-D) The same germarium and early ovariole from a wild-type female stained with anti-EYA (B,C; red in C) and anti-EMC (C,D; green in C). The blue arrowheads indicate intercyst follicle cells in the germarium. The white arrowheads indicate stalk cells between the germarium and a stage-2 egg chamber. The red arrowheads indicate stalk cells between a stage-2 egg chamber and a stage-4 egg chamber. (F,G) The same stage-3 and stage-4 egg chamber and polar/stalk group from a wild-type female stained with anti-Fas3 (red in F) and anti-EMC (F,G; green in F). Arrowheads indicate stalk cells; arrows indicate polar cells. (H) Section of a wild-type stage-6 egg chamber stained with anti-EYA (left panel, and red in middle panel) and anti-EMC (right panel and green in middle panel). Arrowheads indicate a polar cell expressing EMC but not EYA. (I) Posterior of a stage-10 egg chamber possessing a clone of cells (dashed line) that are overexpressing EP3620 under the control of ayGal4. Overexpressing cells are marked with GFP (green). Red indicates EMC. Scale bars: in A, 25 µm in A-G, I; in H, 10 µm in H.

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