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Fig. 4. Loss-of-function phenotypes of emc (A,B,D-Q,S) compared with those
of Notch (C,R,T). Mutant cells are marked by the absence of GFP
(A-E,I-K,M,O,P; green in A-D,I,J,M,O,P). (A-C) clones of cells mutant for
emc1 (A), emcAP6 (B) or
N55E11 (C), stained with anti-Fas3 (red). (D-G) A portion
of the same egg chamber showing a clone of emc1 mutant
cells marked by the loss of GFP (D,E; green in D) expressing elevated Fas3
(D,F; red in D) but not extra PZ80 (D,G; blue in D). Inset in G: PZ80
expression in polar cells from the same experiment. The lines indicate
emc mutant cells. (H,I) A brightfield (H) and a fluorescent section
(I) of the same stage-8 egg chamber possessing a small clone of
emcAP6 mutant cells (arrowhead) marked by the absence of
GFP (I, green) and stained with anti-Cyclin B antibody (I, red). The arrow
indicates yolk. The arrowhead indicates mutant cells magnified in J-L. (J-L)
Magnified region of I, marked with GFP (J,K; green in J), and stained with
anti-Cyclin B antibody (J,L; red in J). Arrowheads indicate an emc
mutant cell expressing Cyclin B. (M,N) Posterior of the same stage-10 egg
chamber, showing a clone of emcAP6 mutant cells (inside
dashed line) and their heterozygous neighbors (green, outside dashed line)
stained with DAPI (M,N; red in M). (O,P) Single sections (O) and a Z-stack (P)
of different egg chambers from animals possessing emc mutant clones
marked with GFP (green) and stained with anti-Fas3 antibody (red). Arrowheads
indicate expected position of polar cells. Arrows indicate polar cells. (Q-T)
Fused egg chambers from different females possessing clones of cells mutant
for emc1 (Q,S) or N55E11 (R,T) stained
with DAPI (Q,R) and with anti-Fas3 antibody (S,T). Arrows indicate oocyte
nuclei; arrowheads indicate polar cells. Scale bars: in A, 10 µm in
A-G,J-P; in H, 25 µm in H,I; in Q, 25 µm in Q-T.
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