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Fig. 3. vab-8 and unc-51 act autonomously in the CAN cell.
vab-8 or unc-51 full-length cDNAs was expressed from the
ceh-23 promoter. At the top is a schematic representation of the
posterior half of C. elegans, showing a CAN cell body and its
posterior axon. Also shown are two sensory neurons (PHA/B) that express the
Pceh-23::gfp transgene and mark the position where the CAN axon
terminates. We scored the extent to which the posterior axons extended, with
the position of the CAN cell body representing 0% extension and the position
of the PHA/B sensory neurons representing 100% extension (see Materials and
methods). The numbers in the boxes represent the percentages of axons that
terminated or turned in that interval. Axons that completed 95-100% of the
distance from the CAN cell body to the sensory neurons are considered wild
type. n is the number of axons scored. To simplify the statistical analysis,
only percentages of the wild-type axons (to the right of the vertical line)
were compared. *The two-tailed Z test was used to compare the
differences in the distributions of the two populations of axons in the
wild-type position.
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