spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. UNC-51 autophosphorylation. (A) Autoradiograph of samples from in vitro kinase assays. FLAG-tagged wild-type and mutant UNC-51 proteins were immunoprecipitated from COS cell extracts and incubated with [{gamma}-32P]ATP. UNC-51(K39R) had 9% and UNC-51({Delta}AIKAI) had 0.6% of the wild-type UNC-51 autophosphorylation activity. See Materials and methods for quantification of kinase activity. (B) Loading control. The western blot was probed with anti-FLAG antibodies to detect various forms of UNC-51 proteins. Similar amounts of immunoprecipitated UNC-51 proteins were used in the kinase assay.





Right arrow Return to article