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Fig. 1. Development of the preplate in 
3 integrin-deficient mice. Neurons
that are destined to the preplate of cerebral cortex were labeled at birth
(E10.5 or E11.5) with BrdU, and their location was analyzed at E17, following
the splitting of the preplate into marginal zone and subplate by invading
migratory neurons. BrdU immunoreactivity was visualized with Cy3 and nuclei
were counterstained with bis benzimide. When neurons were birthdated at E10.5,
in wild-type mice, BrdU-labeled neurons were found in the marginal zone
(arrows) and subplate layers (arrowheads) of cerebral cortex, as expected (A).
Similar distribution was also seen in 3 integrin-deficient cortex (B).
When neurons were birthdated at E11.5, most of the labeled neurons in
wild-type cortex were found in subplate and few in the marginal zone (C).
Identical pattern of labeling is also evident in 3 integrin-deficient
cortex (D). Together, these results indicate that the preplate develops and
splits normally in the absence of 3 integrin. MZ, marginal
zone; CP, cortical plate; SP, subplate; IZ, intermediate zone. Scale bar: 60
µm.
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